| Sparging Supply |
| If you are planning to work with a sparging gas, such as helium, to degas your solvents — or you are considering it — then this section highlights accessories and components you will find useful for this task. |
| Solvent Reservoir |
| The Solvent Reservoir is one of the seven necessary components of any HPLC system. Do you need suggestions regarding various accessories our customers have found useful on the solvent supply side of their systems? Click here to find out more information. |
| Low Pressure Side Of The Pump |
| Delivering the solvent from the solvent reservoir to the pump is often an overlooked section of the HPLC system. This section details some useful components that you will almost certainly want to include as a part of your HPLC system. |
| Pump |
| The HPLC Pump is one of the most complex components of the entire HPLC system, and serves as the second necessary component of any HPLC system. Featuring an array of seals, check valves, pistons, filters and other flow path items, the Pump portion of the system is an area that, if fitted with quality accessories, can provide trouble-free operation for months. |
| High Pressure Side Of The Pump |
| It is in this area of the HPLC system that the fluid undergoes a pressure transition, from the low pressure, suction side of the pump to the high pressure area where the pump is forcing fluid through the packed column. This area requires different tubing and fittings that the low pressure side, and should be coupled with specialized accessories for optimal performance. |
| Injection Valve |
| The Injection Valve — the third main component of a HPLC system — is responsible for introducing the sample into a moving, equilibrated flow path. The proper selection of fittings, tubing ID's, loops and related accessories can ensure proper sample introduction with very little band broadening, thus providing optimal chromatographic results. |
| Between The Injection Valve and Column |
| Now that the sample is introduced to the flow path, it is vitally important to "condition" your sample to ensure that no component of the sample can damage any of the system components. |
| Column |
| Often referred to as the "heart" of the HPLC system, the column is where the needed separation takes place. There are no real accessories that can be used to enhance the performance of the column; the type of column chosen is often the most important determining factor as to the quality of separation achieved. |
| Detector Connections |
| Another of the main HPLC system components, the detector "sees" the sample components. While filters and other accessories are generally not used, choosing the right tubing and fittings can determine if the separation achieved in the column is maintained as the sample components travel to the detector's flow cell. |
| After The Detector |
| What you are doing after the detector will
determine the proper choice of tubing, fittings, and accessories for the
job. Want help dealing with noisy baselines? How about interfacing with
a fraction collector? Click here for suggested products for your needs. |
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