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Tech Center
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Recommendations Moving your mouse over the red hot spots, will bring up a description of that area (listed below graphic). Clicking on that area will take you to its related page. 
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| Sparging Supply |
| If you are planning to work with a sparging
gas, such as helium, to degas your solvents — or you are considering
it — then this section highlights accessories and components you
will find useful for this task. © 2002 Copyright Notice. |
| Solvent Reservoir |
| The Solvent Reservoir is one of the seven
necessary components of any HPLC system. Do you need suggestions regarding
various accessories our customers have found useful on the solvent supply
side of their systems? Click here to find out more information. © 2002 Copyright Notice. |
| Low Pressure Side Of The Pump |
| Delivering the solvent from the solvent reservoir
to the pump is often an overlooked section of the HPLC system. This section
details some useful components that you will almost certainly want to
include as a part of your HPLC system. © 2002 Copyright Notice. |
| Pump |
| The HPLC Pump is one of the most complex components
of the entire HPLC system, and serves as the second necessary component
of any HPLC system. Featuring an array of seals, check valves, pistons,
filters and other flow path items, the Pump portion of the system is an
area that, if fitted with quality accessories, can provide trouble-free
operation for months. © 2002 Copyright Notice. |
| High Pressure Side Of The Pump |
| It is in this area of the HPLC system that
the fluid undergoes a pressure transition, from the low pressure, suction
side of the pump to the high pressure area where the pump is forcing fluid
through the packed column. This area requires different tubing and fittings
that the low pressure side, and should be coupled with specialized accessories
for optimal performance. © 2002 Copyright Notice. |
| Injection Valve |
| The Injection Valve — the third main
component of a HPLC system — is responsible for introducing the
sample into a moving, equilibrated flow path. The proper selection of
fittings, tubing ID's, loops and related accessories can ensure proper
sample introduction with very little band broadening, thus providing optimal
chromatographic results. © 2002 Copyright Notice. |
| Between The Injection Valve and Column |
| Now that the sample is introduced to the flow
path, it is vitally important to "condition" your sample to
ensure that no component of the sample can damage any of the system components. © 2002 Copyright Notice. |
| Column |
| Often referred to as the "heart"
of the HPLC system, the column is where the needed separation takes place.
There are no real accessories that can be used to enhance the performance
of the column; the type of column chosen is often the most important determining
factor as to the quality of separation achieved. © 2002 Copyright Notice. |
| Detector Connections |
| Another of the main HPLC system components,
the detector "sees" the sample components. While filters
and other accessories are generally not used, choosing the right tubing
and fittings can determine if the separation achieved in the column is
maintained as the sample components travel to the detector's flow cell. © 2002 Copyright Notice. |
| After The Detector |
| What you are doing after the detector will
determine the proper choice of tubing, fittings, and accessories for the
job. Want help dealing with noisy baselines? How about interfacing with
a fraction collector? Click here for suggested products for your needs. © 2002 Copyright Notice. |
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